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Flagellar hooks and hook protein FlgE participate in host microbe interactions at immunological level

Scientific Reports,. 2017;

Ying Shen, Lin Chen, Meixiang Wang, Dandan Lin, Zhongjie Liang, Peiqing Song, Qing Yuan, Hua Tang, Weihua Li, Kangmin Duan, Baiyan Liu, Ge Zhao & Yiqiang Wang

Products/Services Used	Details	Operation
Gene Synthesis	The primers were designed according to the genomic accession number AE004901 with addition of restriction enzyme sites NdeI and HindIII at the 5′ ends (forward: GGAATTCCATATGAGTTTCAACATCGGCCTG; reverse: TCCCAAGCTTGCGCAGGTTGATGATGGTCT) and synthesized by GenScript (Nanjing, China).The eluted proteins were run through a His-Trap Desalting Column (GE Healthcare), and then the Toxin EraserTM Endotoxin Removal Kit (GenScript, Piscataway, NJ) was used	Get A Quote