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Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins

Scientific REPORTS. 2017;

Vladimir V. Rogov , Hironori Suzuki, Mija Marinković Verena Lang, Ryuichi Kato, Masato Kawasaki, Maja Buljubašić, Matilda Šprung, Natalia Rogova, Soichi Wakatsuki, Anne Hamacher-Brady, Volker Dötsch, Ivan Dikic , Nathan R. Brady& Ivana Novak

Products/Services Used	Details	Operation
Peptide Synthesis	The 95% pure peptides representing Nix-LIR motif without and with phosphorylation of Ser34 and Ser35 (respectivelly, P0: AGLNSSWVELPMNSSNG, and P2: AGLNpSpSWVELPMNSSNG) were purchased from GenScript USA Inc (N.J., USA). Before the experiment, the proteins and peptides were equilibrated with a buffer containing 40 mM Na2HPO4, 80 mM NaCl at pH 7.0, and supplied with 5 mM protease inhibitors cocktail.	Get A Quote