Distinct activation modes of the Relaxin Family Peptide Receptor 2 in response to insulin-like peptide 3 and relaxin
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| Custom Vector Construction |
Wild-type RXFP2 cDNA with a bovine prolactin signal sequence was inserted in a pcDNA3.1TM/Zeo + AmpR expression vector (Thermo Fisher Scientific) using BamHI and XhoI restriction sites. GB1-RXFP2(1–65) and ssRXFP1 were both inserted into pET15b expression vectors (Novagen Inc.) using NcoI and BamHI restriction sites and NdeI and PstI restriction sites respectively. ssRXFP2 was inserted into a pET28a vector (Novagen Inc.) between NcoI and BamHI sites. GB1-RXFP2(1–65) and ssRXFP2 were produced commercially at Genscript, NJ, USA. |
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