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New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica

Microbial Cell Factories. 2018; 
Marion Trassaert, Marie Vandermies, Fréderic Carly, Olivia Denies, Stéphane Thomas, Patrick Fickers and Jean‑Marc Nicaud
Products/Services Used Details Operation
Catalog Peptides Synthetic promoters carrying three repeated of domains of A box upstream of the wild-type B box (A3B, JMP4123) and the mutated B box (A3b, JMP4124) were synthesised by GenScript Biotech Co. (China) with ClaI and SpeI sites at the 5′ and 3′ ends, respectively Get A Quote

摘要

Background: The oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the produc‑ tion of recombinant proteins. At present, several promoters with different strengths have been developed based either on the constitutive pTEF promoter or on oleic acid inducible promoters such as pPOX2 and pLIP2. Although these promoters are highly efficient, there is still a lack of versatile inducible promoters for gene expression in Y. lipolytica. Results: We have isolated and characterized the promoter of the EYK1 gene coding for an erythrulose kinase. pEYK1 induction was found to be impaired in media supplemented with glucose and glycerol, while the presence of eryth‑ ritol and erythru... More

关键词

Promoter, Regulation, Induction, Synthetic promoter, Erythritol, Erythrulose, Upstream activating sequence, Yarrowia lipolytica, Flow cytometry