Aestivation Induces Changes in the mRNA Expression Levels and Protein Abundance of Two Isoforms of Urea Transporters in the Gills of the African Lungfish, Protopterus annectens.
                    
                    
                    
                    
                        
                            
                                | Products/Services Used | 
                                Details | 
                                Operation | 
                            
                                                            
                                    | PCR Cloning and Subcloning> | 
                                                                            Two rabbit polyclonal antibodies against aa 23– 36 (HNTEKQNDKKESVKC) of Ut-a2a and aa 40–53 (LSENTETSVPEYHPC) of Ut-a2b from P. annectens were custom-made through a commercial company (GenScript, Piscataway, NJ, USA). The concentration of the anti-Uta2a and anti-Ut-a2b antibodies used for Western blotting was 1.7µgml−1.                                         | 
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                            ammonia, nitrogenous waste, urea excretion, Ut-a2a, Ut-a2b