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couples the segmentation clock to somite morphogenesis by regulating N-cadherin-dependent adhesion.

Development. 2017; 
ChalJérome,GuillotCharlène,PourquiéOli
Products/Services Used Details Operation
Custom Vector Construction PAPC RNAi targeting sequences were designed using Genscript and cloned into the RNA interference vector pRFP-RNAi containing a RFP reporter (Das et al., 2006). Get A Quote

摘要

Vertebrate segmentation is characterized by the periodic formation of epithelial somites from the mesenchymal presomitic mesoderm (PSM). How the rhythmic signaling pulse delivered by the segmentation clock is translated into the periodic morphogenesis of somites remains poorly understood. Here, we focused on the role of paraxial protocadherin () in this process. We showed that in chicken and mouse embryos, expression is tightly regulated by the clock and wavefront system in the posterior PSM. We observed that PAPC exhibits a striking complementary pattern to N-cadherin (CDH2), marking the interface of the future somite boundary in the anterior PSM. Gain and loss of function of in chicken embryos disrupt... More

关键词

Boundary formation,Cadherin,Cell adhesion,Endocytosis,Paraxial mesoderm,Protocadherin,Somitogenesis,Tissue morphogen