| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Standard curves were generated by spiking healthy donor serum with 0–100 nM recombinant CFP-10 or ESAT-6, subjected to microwave-assisted digestion and spiked with 10 nM stable isotope-labeled internal standard peptide (m/z 1,603.60 and 1,910.80; GenScript USA), mixed with antibody-conjugated nanodisks for 2 h, pelleted for 5 min at 10,000 ×g, washed three times with 1 mg/mL 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (Avanti Polar Lipids), suspended in 6 μL of deionized water and 4.5 μL (1.5μL ×3) was analyzed by MALDI-TOF-MS, using the MS intensity ratios of each target peptide and internal standards. | Get A Quote |
Tuberculosis (TB) is a major global health threat， resulting in an urgent unmet need for a rapid， non-sputum-based quantitative test to detect active () infections in clinically diverse populations and quickly assess treatment responses for emerging drug-resistant strains. We have identified -specific peptide fragments and developed a method to rapidly quantify their serum concentrations， using antibody-labeled and energy-focusing porous discoidal silicon nanoparticles (nanodisks) and high-throughput mass spectrometry (MS) to enhance sensitivity and specificity. NanoDisk-MS diagnosed active cases with high sensitivity and specificity in a case-control study with cohorts reflecting the complexity of cli... More
