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The mechanism by which a distinguishing arabinofuranosidase can cope with internal di-substitutions in arabinoxylans.

Biotechnol Biofuels. 2018; 
Dos SantosCamila Ramos,de GiuseppePriscila Oliveira,de SouzaFlávio Henrique Moreira,ZanphorlinLetícia Maria,DominguesMariane Noronha,PirollaRenan Augusto Siqueira,HonoratoRodrigo Vargas,TonoliCelisa Caldana Costa,de MoraisMariana Abrahão Bueno,de Matos MartinsVanesa Peixoto,FonsecaLucas Miranda,BüchliFernanda,de OliveiraPaulo Sergio Lopes,GozzoFábio Cesar,MurakamiMário T
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Custom Vector Construction Te DNA sequences of mutants I (containing 249-TVPGPWEKKGPAT-261 and 310-DV-311 instead of 249-TIPGGWPPRASST-261 and 310-AP-311), II (containing 222-NTA-224 instead of 222-SDD-224) and III (mutations I+II) were produced in pET28a vector, between NdeI and XhoI restrictions sites, by GenScript (Piscataway, NJ). Get A Quote

摘要

Arabinoxylan is an abundant polysaccharide in industrially relevant biomasses such as sugarcane, corn stover and grasses. However, the arabinofuranosyl di-substitutions that decorate the xylan backbone are recalcitrant to most known arabinofuranosidases (Abfs).

关键词

Arabinofuranosidase,Arabinoxylan,Crystal structure,Glycoside hydrolase family 51,Molecular mechanism,Oligomeriza