| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … USA). Then, WT and mutated 3′UTR fragments of TGFBR3 were cloned into the pmirGLO vector (Promega, Madison, WI, USA) within PmeI and SalI sites. Both insertions were verified by sequencing (Genscript, Nanjing, China) … | Get A Quote |
Angiogenesis has a great impact on human health， owing to its participation in development， wound healing and the pathogenesis of several diseases. It has been reported that let-7a is a tumour suppressor， but whether it plays a role in angiogenesis is unclear. Here we showed that let-7a， a microRNA conserved in vertebrates， regulated angiogenesis by concomitantly down-regulating TGFBR3. Overexpression of let-7a or knockdown of TGFBR3 in cell culture inhibited the tube formation and reduced migration rate. Moreover， xenograft experiments showed that overexpression of let-7a or knockdown of TGFBR3 had smaller tumour size. Downstream genes， such as VEGFC and MMP9， were also down-regulated in let-7a... More
