| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … The pEGFP-C1 expression vector was from NovoPro (Shanghai, China). HA-Ubiquitin (#18712) and 6myc-p21 (#78780) constructs were from Addgene (Cambridge, MA, USA). The FLAG-SPSP1 expression vector was purchased from GenScript (Piscataway, NJ, USA) … | Get A Quote |
SPRY domain-containing SOCS box protein 1 (SPSB1) is an E3 ligase adaptor protein with unknown functions in cancer cells. In this study， we found that SPSB1 knockdown markedly decreased the viability and migration of ovarian cancer cells， while ectopic SPSB1 overexpression in IL-3-dependent Ba/F3 cells significantly increased their proliferation rate compared with empty vector-transfected cells. SPSB1 knockdown significantly elevated p21 protein and mRNA levels and induced apoptosis in ovarian cancer cells， as evidenced by increased levels of cleaved PARP and decreased levels of Bcl-2. Notably， mechanistic investigations revealed that SPSB1 accelerated p21 destabilization by directly interacting with p2... More
