Macrophages play central roles in immunity as early effectors and modulating adaptive immune reponses; we implicated macrophages in the anticolitic effect of infection with the tapeworm Hymenolepis diminuta. Here， gene arrays revealed that H. diminuta antigen (HdAg) evoked a program in murine macrophages distinct from that elicited by IL-4. Further， HdAg suppressed LPS-evoked release of TNF-α and IL-1β from macrophages via autocrine IL-10 signaling. In assessing the ability of macrophages treated in vitro with an extract of H. diminuta [M] to affect disease， intravenous， but not peritoneal， injection of M protected wild-type but not RAG1 mice from dinitrobenzene sulphonic acid (DNBS)-induced colitis... More
Macrophages play central roles in immunity as early effectors and modulating adaptive immune reponses; we implicated macrophages in the anticolitic effect of infection with the tapeworm Hymenolepis diminuta. Here， gene arrays revealed that H. diminuta antigen (HdAg) evoked a program in murine macrophages distinct from that elicited by IL-4. Further， HdAg suppressed LPS-evoked release of TNF-α and IL-1β from macrophages via autocrine IL-10 signaling. In assessing the ability of macrophages treated in vitro with an extract of H. diminuta [M] to affect disease， intravenous， but not peritoneal， injection of M protected wild-type but not RAG1 mice from dinitrobenzene sulphonic acid (DNBS)-induced colitis. Administration of splenic CD4 T cells from in vitro cocultures with M， but not those cocultured with M cells， inhibited DNBS-induced colitis; fractionation of the T-cell population indicated that the CD4CD25 T cells from cocultures with M drove the suppression of DNBS-induced colitis. Use of IL-4 or IL-10 CD4 T cells revealed that neither cytokine alone from the donor cells was essential for the anticolitic effect. These data illustrate that HdAg evokes a unique regulatory program in macrophages， identifies HdAg-evoked IL-10 suppression of macrophage activation， and reveals the ability of HdAg-treated macrophages to educate ( i.e.， condition) and mobilize CD4CD25 T cells， which could be deployed to treat colonic inflammation.-Reyes， J. L.， Lopes， F.， Leung， G.， Jayme， T. S.， Matisz， C. E.， Shute， A.， Burkhard， R.， Carneiro， M.， Workentine， M. L.， Wang， A.， Petri， B.， Beck， P. L.， Geuking， M. B.， McKay， D. M.， Macrophages treated with antigen from the tapeworm Hymenolepis diminuta condition CD25 T cells to suppress colitis.
