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Gene Synthesis> | … HPLC high performance liquid chromatography. MIC Mininal Inhibitory Concentration. NCBI National Center for Biotechnology Information. PR … synthesis and purified by high performance liquid chromatography using a commercial service (GenScript, Piscataway Township, NJ … | Get A Quote |
Proline-arginine-rich (PR)-39 is neutrophil antimicrobial peptide that has potent antimicrobial activity against a broad spectrum of microorganisms, including bacteria, fungi, and some enveloped viruses as a part of the innate immune system. We analyzed the nucleotide sequence variations of PR-39 exon 4, which is the mature peptide region responsible for antimicrobial activity, from 48 pigs of six breeds using sequence-based typing. The analysis identified four alleles including allele PR-35 with a 12-bp deletion near the N-terminus. Interestingly, 16.7% of individuals showed the presence of three alleles per individual, but only in the Berkshire and Duroc breeds. We further analyzed the genetic d... More