| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … For the construction of stable HER3 overexpression cell line, the pcDNA3/FRT vector (GenScript, China) was used to transfect breast cancer cells MCF-7, which containing the gene of human HER-3, then they were treated by the addition of G418 (20 μg/ml) to co-culture for 3–4 … | Get A Quote |
To explore the relationship between the expression of DJ-1/HER3 and tumor grade in breast cancer， and investigate the effect of HER3 on NRG-1-mediated serum DJ-1 level in vivo. We analyze the expression level of DJ-1 and HER3 in 68 patients with different grades of breast cancer by immunostaining the tissue microarray. Besides， we investigated the serum DJ-1 level by ELISA. We found that the detectable DJ-1 protein expression is decreased， and the HER3 expression is increased in tumor tissue with the progression of breast cancer. There is a significant rise of DJ-1 in serum in vivo with the stimulation of NRG-1. Meanwhile， we found that HER3 knockdown abolishes NRG-1-induced serum DJ-1 increase and HER3... More
