至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Optimization of cyclopropane fatty acids production in Yarrowia lipolytica

Yeast.. 2019-03; 
Czerwiec Q, Idrissitaghki A, Imatoukene N, Nonus M, Thomasset B, Nicaud JM, Rossignol T.
Products/Services Used Details Operation
Gene Synthesis Codon‐optimized CFAs of various organisms (E. coli, Brucella abortus, Pseudomonas putida, Sinorhizobium meliloti, Mycobacterium tuberculosis, Litchi sinensis, and S. foetida) were synthetized by GenScript with extensions to introduce the BamHI or AvrII restriction sites respectively on 5′ and 3′ extremities and cloned into JMP62 vector (JME1047) to provide vectors expressing CFAs under the pTEF promoter listed in Table S1 Get A Quote

摘要

Cyclopropane fatty acids, which can be simply converted to methylated fatty acids, are good unusual fatty acid candidates for long-term resistance to oxidization and low-temperature fluidity useful for oleochemistry and biofuels. Cyclopropane fatty acids are present in low amounts in plants or bacteria. In order to develop a process for large-scale biolipid production, we expressed 10 cyclopropane fatty acid synthases from various organisms in the oleaginous yeast Yarrowia lipolytica, a model yeast for lipid metabolism and naturally capable of producing large amounts of lipids. The Escherichia coli cyclopropane fatty acid synthase expression in Y. lipolytica allows the production of two classes of cyclopropane ... More

关键词