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Gene Synthesis> | Codon‐optimized CFAs of various organisms (E. coli, Brucella abortus, Pseudomonas putida, Sinorhizobium meliloti, Mycobacterium tuberculosis, Litchi sinensis, and S. foetida) were synthetized by GenScript with extensions to introduce the BamHI or AvrII restriction sites respectively on 5′ and 3′ extremities and cloned into JMP62 vector (JME1047) to provide vectors expressing CFAs under the pTEF promoter listed in Table S1 | Get A Quote |
Cyclopropane fatty acids, which can be simply converted to methylated fatty acids, are good unusual fatty acid candidates for long-term resistance to oxidization and low-temperature fluidity useful for oleochemistry and biofuels. Cyclopropane fatty acids are present in low amounts in plants or bacteria. In order to develop a process for large-scale biolipid production, we expressed 10 cyclopropane fatty acid synthases from various organisms in the oleaginous yeast Yarrowia lipolytica, a model yeast for lipid metabolism and naturally capable of producing large amounts of lipids. The Escherichia coli cyclopropane fatty acid synthase expression in Y. lipolytica allows the production of two classes of cyclopropane ... More