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Construction of a recombinant B-cell epitope vaccine based on a Der p1-derived hypoallergen: a bioinformatics approach.

Immunotherapy. 2018-06; 
FanuelSongwe,TabeshSaeideh,MokhtarianKobra,SaroddinyEsmaeil,FazlollahiMohammad Reza,PourpakZahra,FalakReza,KardarGholam
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Proteins, Expression, Isolation and Analysis … and 8 M urea at pH 8.0) for 4 h at 4°C. After solubilization, the sample was centrifuged at 14,000 rpm for 20 min to remove insoluble materials and then the soluble fraction was loaded into 2 ml nickel-nitrilotriacetic acid (Ni-NTA) affinity column (Genscript, NJ, USA), equilibrated … Get A Quote

摘要

House dust mite (HDM) allergens are important elicitors of IgE-mediated allergies. This study was aimed at constructing and characterizing a recombinant fusion protein, DpTTDp, which was based on carrier-bound Der p 1-derived peptides for HDM allergen immunotherapy.

关键词

Der p 1,allergen-specific immunotherapy,allergy,epitopes,house dust mite,tetanus to