| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | … Finally, the suspension was lysed by sonication, and the precipitate and supernatant were separated by centrifuga- tion twice at 8 000 × g and 4°C for 20 min. The supernatant was loaded onto a Ni-NTA affinity column (GenScript, Nanjing, China) … | Get A Quote |
Superoxide dismutase 1 (SOD1) is a metalloenzyme that catalyzes the disproportionation of superoxide into molecular oxygen and hydrogen peroxide. In this study， the human SOD1 (hSOD1) gene was cloned， expressed and purified. The hSOD1 gene was amplified from a pool of Bxpc3 cell cDNAs by PCR and cloned into expression vector pET-28a (+). The recombinant soluble hSOD1 was expressed in E. coli BL21 (DE3) at 37°C and purified using nickel column affinity chromatography. Soluble hSOD1 was produced with a yield of 5.9 μg/mL medium. As metal ions can have a certain influence on protein structure and activity， we researched the influences of different concentrations of Cu and Zn on hSOD1 activity at induction ... More
