| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … serum albumin) was used as the standard. 30 µg protein of each sample was loaded for electrophoresis (140 V, 45 min) in the SDS-PAGE gel (4-20%, ExpressPlus) (Genscript, China). The gel was stained using Coomassie Brilliant Blue R250 (ST031, Beyotime, China) for … | Get A Quote |
Seed storage proteins (SSPs) provide free amino acids and energy for the process of seed germination. Although degradation of SSPs by the aspartic proteases isolated from seeds has been documented in vitro， there is still no genetic evidence for involvement of aspartic proteases in seed germination. Here we report that the aspartic protease ASPG1 (ASPARTIC PROTEASE IN GUARD CELL 1) plays an important role in the process of dormancy， viability and germination of Arabidopsis seeds. We show that aspg1-1 mutants have enhanced seed dormancy and reduced seed viability. A significant increase in expression of DELLA genes which act as repressors in the gibberellic acid signal transduction pathway were detected in a... More
