| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Peptides | … S2) are described in Data S1. USP8 mutant peptides Ser718del, Ser718Cys, Ser718Pro, Pro720Arg, and [Leu713Arg;Tyr717Cys] were purchased from GenScript (Hong Kong) in purity higher than 95%. 14‐3‐3 expression and purification … | Get A Quote |
The ubiquitin-specific protease 8 (USP8)/14-3-3 protein-protein interaction has recently been shown to exert a significant role in the pathogenesis of Cushing's disease (CD). USP8 is a deubiquitinase that prevents epidermal growth factor receptor (EGFR) degradation. Impairment of 14-3-3 binding leads to a higher deubiquitination of EGFR and results in a higher EGFR signaling and an increased production of adrenocorticotropic hormone. Here we report the high-resolution crystal structure of the 14-3-3 binding motif of USP8 surrounding Ser718 in complex with 14-3-3ζ and characterize the interaction with fluorescence polarization and isothermal titration calorimetry. Furthermore， we analyze the effect of USP8 mu... More
