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Human VE-Cadherin Fusion Protein as an Artificial Extracellular Matrix Enhancing the Proliferation and Differentiation Functions of Endothelial Cell.

Biomacromolecules.. 2016-03; 
Xu K, Shuai Q, Li X, Zhang Y, Gao C, Cao L, Hu F, Akaike T, Wang JX, Gu Z, Yang J.
Products/Services Used Details Operation
Catalog Antibody ... collected and used for purification, with target proteins captured on a Protein A CIP 5 9 ml column (GenScript, Cat.No.L00433), followed by a second SEC purification step. 10 ... sample, with primary goat anti-human IgG-HRP utilized (GenScript, Cat. No. 12 A00166). 13 14 ... Get A Quote

摘要

In an attempt to enhance endothelial cell capture and promote the vascularization of engineered tissue, we biosynthesized and characterized the recombinant fusion protein consisting of human vascular endothelial-cadherin extracellular domain and immunoglobulin IgG Fc region (hVE-cad-Fc) to serve as a bioartificial extracellular matrix. The hVE-cad-Fc protein naturally formed homodimers and was used to construct hVE-cad-Fc matrix by stably adsorbing on polystyrene plates. Atomic force microscop assay showed uniform hVE-cad-Fc distribution with nanorod topography. The hVE-cad-Fc matrix markedly promoted human umbilical vein endothelial cells (HUVECs) adhesion and proliferation with fibroblastoid morphology. Addit... More

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