| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | ... 1999). Synthetic plantaricin EF peptides were obtained from Genscript. The concentrations of the peptides in solution were estimated by measuring the absorbance at 280 nm and using an absorbance coefficient calculated from the content of aromatic amino acids. ... | Get A Quote |
Lactobacillus plantarum produces a number of antimicrobial peptides (bacteriocins) that mostly target closely related bacteria. Although bacteriocins are important for the ecology of these bacteria, very little is known about how the peptides target sensitive cells. In this work, a putative membrane protein receptor of the two-peptide bacteriocin plantaricin JK was identified by comparing Illumina sequence reads from plantaricin JK-resistant mutants to a crude assembly of the sensitive wild-type Weissella viridescens genome using the polymorphism discovery tool VAAL. Ten resistant mutants harbored altogether seven independent mutations in a gene encoding an APC superfamily protein with 12 transmembrane helices.... More
