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Study on the biodegradation of crude oil by free and immobilized bacterial consortium in marine environment.

PLoS One.. 2017-03; 
Chen Q,Li J,Liu M,Sun H,Bao M.
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DNA Sequencing ... DNA (1 μL). All the reagents were supplied by Nanjing Genscript Biotechnology Co., Ltd, China. PCR was ... prior to sequencing. The sequencing was completed by Nanjing Genscript Biotechnology Co., Ltd, China. The sequence … Get A Quote

摘要

Five strains of bacteria, namely, Exiguobacterium sp. ASW-1, Pseudomonas aeruginosa strain ASW-2, Alcaligenes sp. ASW-3, Alcaligenes sp. ASS-1, and Bacillus sp. ASS-2, were isolated from the Zhejiang coast in China. The mixed flora of the five strains performed well with degrading 75.1% crude oil (1%, w/v) in 7 days. The calcium alginate-activated carbon embedding carrier was used to immobilize bacterial consortium. Immobilized cells performed better than free ones in variations of environmental factors containing incubated temperature, initial pH, salinity of the medium and crude oil concentration. The degradation process of crude oil by immobilized bacteria was accelerated compared with that of the free ones.... More

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