| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | ... The pET-28b(+) vector was used for assembling the HE expression constructs (GenScript, New Jersey, USA). ... Page 6. flanking the intron insertion sites (~200 nt) were combined, synthesised, and inserted into the pUC57 (2.7 kb) vector (GenScript, New Jersey, USA). ... | Get A Quote |
In vitro characterization of 3 LAGLIDADG-type homing endonucleases (HEs) (I-CcaI, I-CcaII, and I-AstI) that belong to the I-OnuI family showed that they are functional HEs that cleave their respective cognate target sites. These endonucleases are encoded within group ID introns and appear to be orthologues that have inserted into 3 different mitochondrial genes: rns, rnl, and cox3. The endonuclease activity of I-CcaI was tested using various substrates, and its minimum DNA recognition sequence was estimated to be 26 nt. This set of HEs may provide some insight into how these types of mobile elements can migrate into new locations. This study provides additional endonucleases that can be added to the catalog of ... More
