The article studies calmodulin (CaM) as a more universal carrier protein to express many types of AMPs in Escherichia coli. It demonstrates the expression of various antimicrobial peptides (AMPs) using a CaM-fusion expression system, including melittin, fowlicidin-1, tritrpticin, indolicidin, puroindoline A peptide, magainin II F5W, lactoferrampin B, MIP3alpha51-70, and human beta-defensin 3 (HBD-3), the latter requiring three disulfide bonds for proper folding. It proposes the use of the CaM-fusion system as a universal approach to express many cationic amphipathic peptides.
The article studies calmodulin (CaM) as a more universal carrier protein to express many types of AMPs in Escherichia coli. It demonstrates the expression of various antimicrobial peptides (AMPs) using a CaM-fusion expression system, including melittin, fowlicidin-1, tritrpticin, indolicidin, puroindoline A peptide, magainin II F5W, lactoferrampin B, MIP3alpha51-70, and human beta-defensin 3 (HBD-3), the latter requiring three disulfide bonds for proper folding. It proposes the use of the CaM-fusion system as a universal approach to express many cationic amphipathic peptides.
