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Optimization, purification and characterization of recombinant L-asparaginase II in Escherichia coli.

Afr J Biotechnol.. 2016; 
TTH Nguyen, CT Nguyen, TS Le Nguyen, TT Do.
Products/Services Used Details Operation
PCR Cloning and Subcloning ... Plasmid, bacterial strains and cell lines The L-asparaginase gene based on L-asparaginase sequence from GenBank accession number X12746 was optimized codon for expression in E. coli, produced and inserted into vector pUC57 (pUaspg) by GenScript (USA). ... Get A Quote

摘要

Synthetic gene (GenScript, Piscataway, USA) was prepared pursuant to gene aes1 encoding CE16 acetylesterase (Aes1), but without the part coding signal peptide.

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