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Divergent Evolution of Nuclear Localization Signal Sequences in Herpesvirus Terminase Subunits.

J Biol Chem.. 2016-05; 
RS Sankhala, RK Lokareddy, G Cingolani.
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Peptide Synthesis ... Peptides encompassing HSV-1 pUL15 NLS (GPPKKRAKVDVA) and HMCV pUL56 NLS (VSRRVRATRKRPRRAS) were custom synthesized (GenScript) and purified at 95% homogeneity by reverse phase chromatography. A 95% pure peptide comprising SV40 T-antigen NLS ... Get A Quote

摘要

The tripartite terminase complex of herpesviruses assembles in the cytoplasm of infected cells and exploits the host nuclear import machinery to gain access to the nucleus, where capsid assembly and genome-packaging occur. Here we analyzed the structure and conservation of nuclear localization signal (NLS) sequences previously identified in herpes simplex virus 1 (HSV-1) large terminase and human cytomegalovirus (HCMV) small terminase. We found a monopartite NLS at the N terminus of large terminase, flanking the ATPase domain, that is conserved only in α-herpesviruses. In contrast, small terminase exposes a classical NLS at the far C terminus of its helical structure that is conserved only in two genera of the... More

关键词

genome packaging motor; herpesvirus; importin α; nuclear translocation; nuclear transport; terminase; virus assembly; virus entry