| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | ... All primers (Table 1) were synthesized by GenScript Corporation (Piscataway, NJ, USA). Table 1. Rat Primers Used for RT-qPCR(s sense, as antisense). Primer, s/as, Primer sequence 5′ 3′, Gene size. rFcRn, s, CTGTGGATGAAGCAACCTG, 318. as, TCCACGTTTGACCTCTAGC ... | Get A Quote |
PROBLEM: IgG is transformed from maternal serum into the offspring as passive immunization by FcRn expressed in placenta and/or infant intestine. This study aimed to investigate the quantitative correlation between IgG and FcRn during gestation and lactating periods in rat. METHOD OF STUDY: ELISA was performed to determine the variation of maternal and offspring IgG. Western blot and RT-qPCR were carried out to characterize FcRn expression in placenta and infant intestine. RESULTS: Maternal serum IgG appeared higher in first 2 weeks of lactation. The embryo IgG was in accordance with the FcRn expression in placenta. During the post-natal, the serum IgG concentration in feta was obviously lower than maternal ... More
