To accelerate development of pea cultivars with improved nutritional profiles, information about allelic variations within genes for major seed composition in diverse pea accessions is required. The objective of this study was to identify single nucleotide polymorphisms (SNPs) within genes associated with amylose, total starch and crude protein concentration in seeds. Fifty diverse Pisum sativum accessions collected from eastern Europe, western Europe, Australia, and Canada were used to determine amylose, total starch and crude protein concentration. Thirteen published genes associated with carbohydrate metabolism and seed storage protein deposition were amplified from these accessions. One hundred thirty two S... More
To accelerate development of pea cultivars with improved nutritional profiles, information about allelic variations within genes for major seed composition in diverse pea accessions is required. The objective of this study was to identify single nucleotide polymorphisms (SNPs) within genes associated with amylose, total starch and crude protein concentration in seeds. Fifty diverse Pisum sativum accessions collected from eastern Europe, western Europe, Australia, and Canada were used to determine amylose, total starch and crude protein concentration. Thirteen published genes associated with carbohydrate metabolism and seed storage protein deposition were amplified from these accessions. One hundred thirty two SNPs were detected within these genes. Out of 118 SNPs associated with carbohydrate metabolism, four and ten SNPs were significantly (P < 0.05) associated with amylose in either 2008 or 2009, and total starch concentration in the majority of station years, except Spsp141 which was significant in four of eight station years, respectively. One SNP was significantly associated with crude protein concentration in five of eight station years. Identified SNPs have the potential for use in marker assisted selection in pea breeding.
