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Stable, Site-Specific Fluorescent Tagging Constructs Optimized for Burkholderia Species.

Appl Environ Microbiol.. 2010-11;

Michael H. Norris, Yun Kang, Bruce Wilcox, and Tung T. Hoang. Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.

Products/Services Used	Details	Operation
Gene Synthesis	...mini-Tn7 plasmid backbone is based on pUC18R6KT-mini-Tn7T (7). Fluorescent protein genes (cfp, rfp, yfp) were synthesized by GenScript and cloned into the EcoRV site of pUC57. These genes were removed by digestion with KpnI and PstI and then subcloned into...	Get A Quote

摘要

Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.