目录产品 » HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus macaque
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HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus macaque

HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
￥9900
Z06679-100

产品简介
Species Rhesus macaque
Protein Construction
HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer [Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide]
Accession # O02948(HLA-G)&Q6V7J5(B2M)&RIIPRHLQL		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus macaque at 5μg/ml (100μl/well) on the plate can bind Rhesus macaque LILRB1, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 258 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus Macaque

Immobilized HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus macaque, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 102 ng/ml determined by ELISA. »

HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus Macaque

The purity of HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus macaquer is greater than 95% as determined by SEC-HPLC. »

HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus Macaque

HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus macaque on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%. »

HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus Macaque

Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His & Avi, Rhesus macaque, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Synonyms HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.