| Species |
Mouse |
| Protein Construction |
Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer[ His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide]
Accession # P06339(Q1-1b)&P01887(B2M)&AMAPRTLLL |
His |
Avi |
| N-term |
|
C-term |
|
| Purity |
> 95% as determined by TrisBis PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
50.60 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Qa-1b binds a peptide (AMAPRTLLL), referred to as Qdm (for Qa-1 determinant modifier), derived from the signal sequence of murine class Ia molecules. This peptide binds with high affinity and accounts for almost all of the peptides associated with this molecule. Human histocompatibility leukocyte antigen (HLA)-E, a homologue of Qa-1b, binds similar peptides derived from human class Ia molecules and interacts with CD94/NKG2 receptors on natural killer cells. |
| Synonyms |
Qdm; Qa-1b; MHC; H2-T23 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
