目录产品 » HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human
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HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human

The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
￥3000
Z06598-100

产品简介
Species Human
Protein Construction
HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer[Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide]
Accession # A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human at 2μg/ml (100μl/well) on the plate can bind Anti­HLA­A*02:01&B2M&WT­1 (RMFPNAPYL) Antibody, hFc Tag. Test result was comparable to standard batch
Expression System HEK293
Theoretical Molecular Weight 50.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human

Immobilized HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 Antibody, hFc Tag with the EC50 of 11.2 ng/ml determined by ELISA. »

HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human

The purity of HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human was greater than 95% as determined by SEC-HPLC. »

HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human

HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human

HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His & Avi, Human, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with an affinity constant of 0.33nM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
Synonyms MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.