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| Z06592-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His & Avi, Human at 2μg/ml (100μl/well) on the plate can bind HLAA*02:01&B2M&PRAME TCR. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 50.40 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 51-58 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 55.4 ng/ml determined by ELISA. »
The purity of HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited. |
| Synonyms | PRAME; OIP-4; OIP4; MAPE |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
