HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.

价格	￥9900 在线订购包邮(港澳台除外)
产品编号	Z06559-100
规格	100 μg 500 μg 大单询价
数量
参考货期


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产品简介图例技术背景

产品简介

Species

Human

Protein Construction

HLA-A02:01&B2M&GP100 (YLEPGPVTA) Tetramer [Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide] Accession # A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA	His	Avi
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

258 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

图例

Immobilized HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 Antibody, hFc Tag with the EC50 of 5.6 ng/ml determined by ELISA. »

The purity of HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human was greater than 95% as determined by SEC-HPLC. »

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%. »

Immobilized Tebentafusp, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human, His Tag with the EC50 of 39.5 ng/ml determined by ELISA. »

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human, His Tag immobilized on CM5 Chip can bind Tebentafusp with an affinity constant of 0.196 nM as determined in SPR assay (Biacore T200). »

技术背景

Target Background	Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
Synonyms	HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.