目录产品 » HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human
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HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human

HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
￥14800
Z06512-100

产品简介
Species Human
Protein Construction
HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer [Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide]
Accession # P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate can bind AntiB2M Antibody, mFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 258 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human

Immobilized HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/Well) on streptavidin(5 μl/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.7 ng/ml determined by ELISA. »

HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human

The purity of HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human

HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%. »

HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human

Serial dilutions of Anti-NKG2A Antibody were added into HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer[Biotin], His & Avi, Human, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 0.15 μg/ml. »

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技术背景
Target Background HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
Synonyms HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.