| ￥4500 | |
| Z06473-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer[Biotin], His & Avi, Human at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind AntiHLAA*02:01&B2M&MAGEA4 (GVYDGREHTV) Antibpdy, hFc Tag. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 50.5 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer[Biotin], His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 Antibody, hFc Tag with the EC50 of 3.0 ng/ml determined by ELISA. »
The purity of HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer[Biotin], His & Avi, Human was greater than 95% as determined by SEC-HPLC. »
HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) Complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4. |
| Synonyms | HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
