Species | Human | ||||||
Protein Construction |
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Conjugate | Biotin | ||||||
Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate can bind Anti-B2M Antibody, mFc Tag. Test result was comparable to standard batch. | ||||||
Expression System | HEK293 | ||||||
Theoretical Molecular Weight | 50.50 kDa | ||||||
Apparent Molecular Weight | Due to glycosylation, the protein migrates to 53-65 kDa based on Bis-Tris PAGE result. | ||||||
Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||||
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 6.8 ng/ml determined by ELISA. »
The purity of HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Target Background | MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors. |
Synonyms | MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.