Immobilized HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 Antibody, hFc Tag with the EC50 of 5.6 ng/ml determined by ELISA.

The purity of HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer[Biotin], His & Avi, Humanr was greater than 95% as determined by SEC-HPLC.

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer[Biotin], His & Avi, Human

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I and YLEPGPVTA peptide of the gp100.

产品编号	Z06463
规格	100 μg 500 μg 大单询价
数量
价格	￥4500


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产品简介

Species

Human

Protein Construction

HLA-A02:01&B2M&GP100 (YLEPGPVTA) Monomer [Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide] Accession # A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer[Biotin], His & Avi, Human at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind AntiHLAA*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

50.4 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

The purity of HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer[Biotin], His & Avi, Humanr was greater than 95% as determined by SEC-HPLC.

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A0201 of the MHC Class I and YLEPGPVTA peptide of the gp100.
Synonyms	HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer[Biotin], His & Avi, Human

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