目录产品 » HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer[Biotin], His & Avi, Human
Manual
SDS
COAs

HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer[Biotin], His & Avi, Human

Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.
￥4500
Z06462-100

产品简介
Species Human
Protein Construction
HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer (Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide)
Accession # A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE
> 95%  as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized HLA-A*02:&B2M&CMVpp65 (NLVPMVATV) Monomer[Biotin], His & Avi, Human at 1μg/ml(100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Anti-B2M Antibody, mFc Tag. Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M)
Apparent Molecular Weight The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
返回

图例
HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer[Biotin], His & Avi, Human

Immobilized HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer[Biotin], His & Avi, Human, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.1 ng/ml determined by ELISA. »

HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer[Biotin], His & Avi, Human

The purity of HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer[Biotin], His & Avi, Human

HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

<
>

技术背景
Target Background Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02?, the most common MHC-I allele in the population.
Synonyms CMVpp65; NLVPMVATV
返回

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.