| Species |
Rat |
| Protein Construction |
| His |
PDGF-AA (Arg86-Lys204)_x000D_
Accession # P28576-1 |
| N-term |
C-term |
|
| Purity |
> 90% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 5 15 ng/mL. Test result was comparable to standard batch. |
| Expression System |
Yeast |
| Theoretical Molecular Weight |
14.75 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 17-22 kDa and 25-45 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
PDGF (Platelet-derived growth factor) is one among numerous growth factors that regulate cell growth and division. The PDGF family forms five dimeric ligands, PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD, and PDGF-AB, which activate cognate receptor tyrosine kinases PDGFR-α and PDGFR-β. Upon binding to its tyrosine kinase receptor PDGF-αα, PDGF-AA stimulates the proliferation of osteoblastic cells and may exert autocrine and paracrine effects in regulating bone-forming processes. PDGF-AA is the most well characterized mitogen that is not restricted to any kind of tumor cell type but was seen in osteoblastic cells as well as in pleomorphic tumor elements. |
| Synonyms |
PDGF-AA; PDGF-1; Pdgfa; PDGF-A; PDGF1; PDGF A; PDGFAA; PDGF AA; Rpa1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
