| ￥1300 | |
| Z06194-100 | |
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| Species | Mouse | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized IL-18BP, His, Mouse at 1μg/ml (100μl/well) on the plate can bind Biotinylated IL18, No Tag. Test result was comparable to standard batch | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 19.1 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 45-65 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized IL-18BP, His, Mouse, His Tag at 1 μg/ml(100 μl/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 21.5 ng/ml determined by ELISA. »
IL-18BP, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
IL-18BP, His, Mouse, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.86 nM as determined in SPR assay (Biacore T200). »
| Target Background | Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice. |
| Synonyms | IL-18BP; Igifbp |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
