| ￥3000 | |
| Z06131-100 | |
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| Species | Mouse | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 26 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A. »
Immobilized Human Activin A, No Tag at 0.2 μg/ml (100 μI/well) on the plate. Dose response curve for FSTL3, His, Mouse, His Tag with the EC50 of 20.1ng/ml determined by ELISA. »
The purity of FSTL3, His, Mouse is greater than 95% as determined by SEC-HPLC. »
FSTL3, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
FSTL3, His, Mouse, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A, No tag with an affinity constant of 53.09 pM as determined in SPR assay (Biacore T200). »
| Target Background | Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD. |
| Synonyms | FLRG; FLRGFSRP; Follistatin-like 3; FSTL3 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
