| ￥3500 | |
| Z06088-100 | |
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| Species | Mouse | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1 EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 18.79 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Loaded Mouse TNFSF15, monomeric Fc Tag on ProA-Biosensor, can bind DR3/TNFRSF25, His, Mouse, His Tag with an affinity constant of 22.80 nM as determined in BLI assay (Gator). »
Immobilized DR3/TNFRSF25, His, Mouse, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His-Avi Tag with the EC50 of 4.9ng/ml determined by ELISA. »
The purity of DR3/TNFRSF25, His, Mouse is greater than 95% as determined by SEC-HPLC. »
DR3/TNFRSF25, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind DR3/TNFRSF25, His, Mouse, His Tag with an affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). »
| Target Background | Death Receptor 3 (DR3), also known as TNFRSF25, TRAMP, LARD, or WSL-1, is a death-domain-containing TNF-family receptor that, like its closest paralog TNFR1, binds the adaptor molecule TRADD through its cytoplasmic death domain. TRADD recruitment endows DR3 with dual-signaling capability to activate NF−κB and MAP-kinase signaling or alternatively trigger caspase activation and programmed cell death. |
| Synonyms | APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; TNFRSF25; LARD; TR3; TRAMP; WSL-LR |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
