APRIL/TNFSF13 hFc Chimera, Mouse
| ¥3500 | |
| Z06013-100 | |
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| ¥3500 | |
| Z06013-100 | |
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| Species | Mouse | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 43.20 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind APRIL/TNFSF13 hFc Chimera, Mouse, hFc Tag with an affinity constant of 7.09 nM as determined in BLI assay (Gator® Prime). »
Immobilized APRIL/TNFSF13 hFc Chimera, Mouse, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 5.4/5.1ng/ml determined by ELISA. »
The purity of APRIL/TNFSF13 hFc Chimera, Mouse is greater than 95% as determined by SEC-HPLC. »
APRIL/TNFSF13 hFc Chimera, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. |
| Synonyms | CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.