| Species |
Human |
| Protein Construction |
ULBP-4 (His31-Asp225)_x000D_
Accession # Q8TD07-1 |
hFc |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
ULBP-4 hFc Chimera, Human captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag in SPR assay (Biacore T200). Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
49.12 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
UL16-binding proteins (ULBPs) are markers of cellular stress which are upregulated on the surface of virus-infected and tumor cells. ULBPs are expressed by a variety of leukemias, carcinomas, melanomas, and tumor cell lines.ULBP4 is associated with higher levels of CD8+/NKG2D+ lymphocytes infiltrating the carcinoma and is a prognosticator of improved survival. |
| Synonyms |
N2DL-4; NKG2DL4; RAE-1-like transcript 4; RAET1E; LETAL; N2DL4; ULBP4; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
