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Transferrin, His, Human

Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.
￥1600
Z05917-100

产品简介
Species Human
Protein Construction
Transferrin (Val20-Pro698)_x000D_
Accession # AAH59367		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 76.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 78-82 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
Transferrin, His, Human

Immobilized Transferrin, His, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 0.27 μg/ml determined by ELISA. »

Transferrin, His, Human

The purity of Transferrin, His, Human is greater than 95% as determined by SEC-HPLC. »

Transferrin, His, Human

Transferrin, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Transferrin, His, Human

Transferrin, His, Human, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 11.26 nM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.
Synonyms Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.