| ￥3300 | |
| Z05902-100 | |
|
|
|
|
|
|
|
|
|
| Species | Human | ||||
| Protein Construction |
|
||||
| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 49.62 kDa | ||||
| Apparent Molecular Weight | Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized TNFSF12/TWEAK hFc Chimera, Human, hFc Tag at 5 μg /ml (100 μl /well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc-Avi Tag with the EC50 of 0.22 μg /ml determined by ELISA. »
The purity of TNFSF12/TWEAK hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
TNFSF12/TWEAK hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
TNFSF12/TWEAK hFc Chimera, Human, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). »
| Target Background | Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation. |
| Synonyms | APO3 ligand; APO3L; DR3LG; UNQ181/PRO207; TWEAK; MGC129581 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
