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Siglec-10, His, Human

Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
￥3500
Z05817-100

产品简介
Species Human
Protein Construction
Siglec-10 (Met17-Thr546)_x000D_
Accession # Q96LC7-1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Siglec-10, His, Human at 1μg/ml (100μl/Well) on the plate can bind Anti­Siglec­10 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 59.2 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 70-79 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM PB, 150mM NaCl, 25mM L-arginine (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
Siglec-10, His, Human

Immobilized Siglec-10, His, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. »

Siglec-10, His, Human

The purity of Siglec-10, His, Human is greater than 95% as determined by SEC-HPLC. »

Siglec-10, His, Human

Siglec-10, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Siglec-10, His, Human

Siglec-10, His, Human, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CD52, mFc Tag with an affinity constant of 15.9μM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Synonyms SLG2; SIGLEC10; MGC126774; PRO940
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.