| Species |
Human |
| Protein Construction |
R spondin 3/RSPO3 (Gln22-Val146)_x000D_
Accession # Q9BXY4-1 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized R spondin 3/RSPO3, His, Human at 1μg/ml (100μl/well) on the plate can bind Human RNF43, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
14.95 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 20-25 kDa and 26-32 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein. |
| Synonyms |
R-spondin-3; RSPO3; hPWTSR; hRspo3; PWTSR; THSD2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
