| ￥2600 | |
| Z05745-100 | |
|
|
|
|
|
|
|
|
|
| Species | Human | ||||
| Protein Construction |
|
||||
| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | PD-L2/B7-DC hFc Chimera, Human captured on CM5 Chip via Protein A can bind Human PD1, His Tag in BLI assay (Gator® Prime). Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 48.60 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
The purity of PD-L2/B7-DC hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
PD-L2/B7-DC hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
PD-L2/B7-DC hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 0.21 μM as determined in SPR assay (Biacore T200). »
| Target Background | PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals. |
| Synonyms | PDL2; PD-L2; Butyrophilin B7-DC; CD273, PDCD1 ligand 2; PDCD1L2; PDCD1LG2; B7DC; bA574F11.2; Btdc |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
