| ￥3000 | |
| Z05725-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 40.95 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized Human OX40, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for OX40 Ligand/TNFSF4 hFc Chimera, Human, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. »
The purity of OX40 Ligand/TNFSF4 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
OX40 Ligand/TNFSF4 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind OX40 Ligand/TNFSF4 hFc Chimera, Human, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). »
| Target Background | Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. |
| Synonyms | OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L; TXGP1; Glycoprotein Gp34 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
